Multiple & Portable ELISA
Patents: EP 1 499 894 B1 - USPTO 7,510,687 - ZL 03 8 10029.0 - PCT/IT03/00218 - WO 03/085401
The innovation and its advantages
Multiple & Portable ELISA is based on patented innovative method and device:
the multi-catcher device with 8 or 12 immunosorbent ogival pins, held in a special rack suitable for moving 8 or 12 multi-catcher devices, easily and simultaneously.
Device pins are sensitized with different catchers and different tests are carried out in one shot.
No Lab equipments are needed for:
- distributing samples: the multi-catcher device is immerged and incubated in the tube where the sample was collected;
- carrying out washing steps: washing can be made using microshower, dispenser, tap water or pre-filled microplates;
- distributing conjugate and chromogenic substrate: pre-filled 96 well plates with the needed reagents are used, sealed and stored until their use;
- kits for 8 or 12 samples contain 1 microplate pre-filled with conjugate and 1 microplate pre-filled with chromogenic substrate;
- kits for single sample contain only 1 microplate pre-filled with washing solution, conjugate and chromogenic substrate in different columns.
Sample enrichment and sample concentration are not needed: the tested sample aliquot increases, easing the highly diluted target detection.
System Components
Performing the Test
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1. dip a multi-catcher device in the collected
sample and incubate extract the device and wash it |
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2. assemble the devices in the rack, and dip the pins into the conjugate contained in a pre-filled microplate and incubate |
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extract the rack and wash the pins | |
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3. dip the pins into the chromogenic substrate contained in a pre-filled microplate and incubate then take out the rack and… |
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| 4. ... DONE!!! Your multiple tests are ready for reading | ||
Reading can be performed visually, for screening purposes, or using the commercially available ELISA-readers (TMB: λ = 655 nm; λ = 450 nm when stop solution is added)
Multiple & Portable ELISA Applications
Beyond the laboratory boundaries, cheaply
Multiple & Portable ELISA is specially fitted for optimizing and commercializing:
- ELISA ready-to-use lab kits
- ELISA on-site kits
ELISA ready-to-use lab kits and ELISA on-site kits don’t need skilled personnel and lab equipments, so that:
- cheap labs can be spread widely in:
- not served areas
- small industries
- Med and Vet Offices
- ……..
- large population can be screened in situ during:
- sanitary alerts
- sport competitions
- epidemiological surveys in not well established economies
- duty controls
- …..
in the aim of:
- reaching new end-users
- extending screening applications
Applications
In a global scenario, the need of increasing controls can be satisfied joining easiness and handling to the ELISA high sensitivity and specificity: ready-to-use lab kits and on-site kits based on Multiple & Portable ELISA are suitable in:
Human Health
- Breathing Infectious Diseases
- Sexually Transmitted Infectious Diseases
- Enteric Infectious Diseases
- Sanitary Alerts
- Cancer markers
- Anti-Doping
Food Safety
- Pathogens
- Allergens
- Bacterial Toxins
- Mycotoxins
- Anabolic agents
- Antibiotics
- Pesticides
- GMO
- Species identification
Animal Health
- Anti-Doping (racecourse)
- Animal Infectious Diseases and Zoonoses (livestock)
- Animal Infectious Diseases and Zoonoses (pets)
Plant Cultivation
- Plant Infectious Diseases
- GMO
Water Quality
- Pesticides
- Pathogens
- Bioterrorism agents
