Surfaces Selection Guide

surface	code	binding capacity	binding interaction	proteins properties	suggested uses
Medium Binding	MB	100 ng IgG/cm²	hydrophobic	high molecular weight	Monoclonal antibodies / proteins with large hydrophobic regions
High Binding	HB8	400 ng IgG/cm²	hydrophobic and ionic (-)	medium to high molecular weight	Assays where the adsorbed molecule must work in excess (up to 400 ng/cm²) - e.g. ELISA determination for lgG (indirect ELISA test or ELISA capture test for lgM detection. Very suitable to set up competitive tests (molecule <50 ng/cm²) - e.g. steroids/ hormones - for the ability to orientate the adsorbed molecule.
No Binding	NB	0	none		Proteins which must not react with the surface
Streptavidin coated	SA 5/200	18 pmol of biotin	streptavidin/biotin non covalent biological strongest interaction	any biotinylated protein	Proteins- peptides- polysaccharides- oligonucleotides- DNA fragments When it is necessary a great increase of the sensitivity of the assay
Protein A coated	PA		specific for Fc of IgG	IgG	Binds the Fc region of Immunoglobulin molecules without interacting at the antigen binding site
Protein G coated	PG		specific for Fc of IgG	IgG	Binds the Fc region of Immunoglobulin molecules without interacting at the antigen binding site
Concanavalin A coated	Con A		specific for C3-C4-C5 hydroxyl groups	carbohydrates	High affinity for terminal -D-mannosyl and -D-glucosyl residues. Used to immobilise glycoproteins or carbohydrates containing these groups
Jacalin coated	Jac		high affinity for _D-galactose residues of the biochemical structure of IgA1 and cell membranes		Human IgA1 specific binding, sterically oriented Purification of human immunoglobulins (especially IgA1) Separation of immunocomplexes antigen-antibody Separation of IgA1 from contaminants Stimulation of T-cells
Wheat Germ coated	WG		specific binding of N-acetyl-D-glucosamine residue of the biochemical structure of glycoproteins, enzymes and cell membranes		Studies of surfaces of normal and transformed cells Glycoprotein purification including membrane glycoproteins Studies of cell surface changes during development and the cell cycle
Poly-L-Lysine coated	LYS-L		high density of: α-amino/ α-carboxyl/ ε-amino groups able to react trough electrostatic and stereospecific bonds		Interactions with plasminogen and plasminogen activator Interactions with ribosomial RNA Interactions with double stranded DNA
Poly-L-Arginine coated	AR		high density of: α-amino/ α-carboxyl/ guanidino groups able to react trough electrostatic and stereospecific bonds		Interactions with serino proteases Interactions with maturation promoting factors
Calmodulin	Cal		hydrophobic sites		Interactions with proteins involved in glycogen metabolism Interactions with factors involved in neurotransmission mechanism Interactions with enzymes involved in the NAD/NADP phosphorilation system
Biotin	Bio		streptavidin/biotin non covalent biological strongest interaction		Interactions with avidin Interactions with streptavidin
Aminated with primary amines and secondary amines	AM 1 AM 2	covalent via crosslinkers	molecules with functional groups as amine-carboxyl -sulphydryl		Immobilisation of molecules which are bound weakly or not at all by physical adsorption, namely small peptides (M.W. 1000-5000) drugs, toxins or hormones. Oriented immobilisation of molecules in order to secure the integrity and accessibility of their specific sites avoiding the risk of inhibition of these sites by casual physical adsorption for such molecules as Fab-SH-antibody fragments, or nucleic acids (single strand or double strand)
Carboxylated	COOH		covalent via crosslinkers	molecules with an amino group on their structure	The amino group presents in any molecules, such as peptides or proteins, binds to COOH surface through formation of amide bonds between the amino group presents in the molecule and the surface carboxylic group by the action of carbodiimide.